GUANINE NUCLEOTIDE BINDING PROTEINS AS RECEPTOR-EFFECTOR COUPLERS

Project: Research project

Project Details

Description

A family of guanine nucleotide binding proteins (G-proteins) functions
in transmembrane signalling as receptor-effector couplers. G-proteins
couple to a diverse array of receptors including those for hormones,
neurotransmitters, light odorants, and certain growth factors. Effector
functions regulated (positively and, in some instances, negatively) by
G-proteins include cAMP formation, phosphoinositide breakdown, potassium
and calcium channels, and cGMP degradation. We have used a variety of
techniques to study the expression, distribution, regulation, structure
and function of G-proteins. Our studies highlight the diversity within
the G-protein family. Using peptide specific antibodies, we have defined
the specificity of G-proteins in coupling to receptors and effectors.
We have defined distinct post-translational lipid modifications necessary
for membrane attachment of G protein alpha and beta/gamma subunits. We
have created mutations in alpha subunits that cause constitutive
activation, and transfected these into cells to define phenotypic effects
on cellular function. These studies provide the basis for understanding
the role of G-proteins in normal signal transduction and for elucidating
possible defects in G-protein structure or function as the basis for
abnormal signal transduction.
StatusNot started

Funding

  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases

ASJC

  • Genetics
  • Molecular Biology
  • Cell Biology

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