GUANINE NUCLEOTIDE BINDING PROTEINS AS RECEPTOR-EFFECTOR COUPLERS

Project: Research project

Project Details

Description

A family of guanine nucleotide binding proteins (G-proteins) functions
in transmembrane signalling as receptor-effector couplers. G-proteins
couple to a diverse array of receptors including those for hormones,
neurotransmitters, light, odorants, and certain growth factors. Effector
functions regulated (positively and, in some instances, negatively) by
G-proteins include cAMP formation, phosphoinositide breakdown, potassium
and calcium channels, and cGMP degradation. We have used a variety of
techniques to study the expression, distribution, regulation, structure
and function of G-proteins. Our studies highlight the diversity within
the G-protein family. Using peptide specific antibodies, we have defined
the specificity of G-proteins in coupling to receptors and effectors.
We have created mutations in alpha subunits that cause constitutive
activation, and transfected these into cells to define phenotypic effects
on cellular function. We have identified mutations in G proteins and in
G protein-coupled receptors as the basis of several human diseases.
These studies provide the basis for understanding the role of G-proteins
in normal signal transduction and for elucidating possible defects in G-
protein structure or function as the basis for abnormal signal
transduction.
StatusNot started

ASJC

  • Cell Biology