Following Single Messenger RNA Molecules from Birth to Death in Eukaryotic Cells

Project: Research project

Project Details

Description

? DESCRIPTION (provided by applicant) Over the last funding period, we have made a paradigm-shifting observation: that the information contained in the promoter dictates the fate of the mRNA in the cytoplasm. Hence mRNA fate in the cytoplasm can be determined at the time of its birth. This remote control regulation of the mRNA outcome appears to apply to genes with time dependent expression patterns, such as cell cycle, metabolic product or stress induced genes. We propose to uniquely integrate biochemical, genetic and imaging approaches to identify the hierarchy of factors imprinted co-transcriptionally in the mRNA as well as to define the regulatory DNA sequences that dictate this mRNA behavior. The use of innovative technology, combining the use of microscopy with fluorescent tagging of mRNA is directed toward elucidating the coordination of transcription and downstream events of the RNA life; events that likely create an autoregulatory loop. We envision a tight coupling from transcription through decay and back, mediated by specific factors that would be released from the mRNA upon its degradation, to signal the gene to make more. We hypothesize that cell cycle and stress-regulated genes require a robust coordination between transcription, translation and decay. We expect that factors imprinted by the promoter regulate the strength of the transcriptional response. Taken together, these observations suggest a shuttling of factors between the nucleus and the cytoplasm important for fine-tuning the response of the cell. The Aims of this project chart a straightforward path to investigate the mechanisms regulating the fate of an mRNA during transcription and how the cells achieve precise gene expression patterns by coordinating mRNA transcription, translation and decay. In order to do this, we will develop and use tools specifically reporting each of the steps in mRNA metabolism and identify factors that coordinate these steps. 1. Impact of promoter sequence on transcription and mRNA localization 2. Purification of factors regulating transcription and mRNA fate 3. Visualization of proteins loaded on the nuclear transcripts and followed into the cytoplasm 4. Kinetics of translation and decay and factors
StatusFinished
Effective start/end date1/1/9811/30/19

Funding

  • National Institute of General Medical Sciences: $300,600.00
  • National Institute of General Medical Sciences: $320,956.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $360,879.00
  • National Institute of General Medical Sciences: $265,357.00
  • National Institute of General Medical Sciences: $310,747.00
  • National Institute of General Medical Sciences: $48,575.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $258,586.00
  • National Institute of General Medical Sciences: $320,620.00
  • National Institute of General Medical Sciences: $328,305.00
  • National Institute of General Medical Sciences: $317,747.00
  • National Institute of General Medical Sciences: $119,645.00
  • National Institute of General Medical Sciences: $300,600.00
  • National Institute of General Medical Sciences: $319,500.00
  • National Institute of General Medical Sciences: $328,305.00
  • National Institute of General Medical Sciences: $80,155.00
  • National Institute of General Medical Sciences: $279,730.00
  • National Institute of General Medical Sciences: $240,465.00
  • National Institute of General Medical Sciences: $293,536.00
  • National Institute of General Medical Sciences: $328,305.00
  • National Institute of General Medical Sciences: $300,600.00
  • National Institute of General Medical Sciences: $309,398.00

ASJC

  • Genetics
  • Molecular Biology
  • Biochemistry

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