ERBB1 AND ERBB2 IN TUMOR MOTILITY AND METASTASIS

Project: Research project

Project Details

Description

DESCRIPTION: (Adapted from the investigator's abstract) The proteins ErbB1 and
ErbB2 have been found to be important indicators of poor prognosis in breast
cancer. However, the mechanisms by which these molecules contribute to
metastasis have not been established. In particular, determination of whether
there is a rate limiting step for metastasis which is determined by these
molecules is unknown. Identification of the stage of metastasis that is
enhanced by ErbB1 and ErbB2 together with the molecular mechanism is critical
for determining approaches for treatment and prognosis of breast cancer. He has
developed new methods for evaluating tumor cells in the primary tumor, blood
and target organs, and will use these methods for evaluating tumor cells in the
primary tumor, blood and target organs, and will use these methods to provide
important information regarding how ErbB1 and ErbB2 function in metastasis. The
objective of this proposal is to determine whether chemotaxis stimulated by
ErbB1 and ErbB2 is rate-limiting for metastasis. The first specific aim will be
to complete development of a metastasis assay that will provide information of
blood burden, cell arrest in the lungs and metastasis formation in the lungs. A
pair of well-characterized rat mammary adenocarcinoma cell lines using
orthotopic implantation in a syngeneic host are being used to develop the
assay. The assay will then be applied to human mammary adenocarcinoma cell
lines implanted in nude mice. The second specific aim will be to use this assay
to evaluate the contributions of ErbB1 and ErbB2 to metastasis in terms of
chemotaxis and mitogenesis. The third specific aim will evaluate the roles of
the rho family of small G proteins in metastasis.
StatusFinished
Effective start/end date2/15/001/31/01

Funding

  • National Cancer Institute: $301,875.00

ASJC

  • Molecular Medicine
  • Oncology
  • Cancer Research
  • Cell Biology
  • Histology

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