Engineering Sortase Variants for Intracellular and Cell Surface Labeling

  • Levy, Matthew (PI)

Project: Research project

Project Details

Description

DESCRIPTION (provided by applicant): We propose to develop a new system for site specific labeling proteins in live cells based on the bacterial transpeptidase sortase A (SrtA). Using a combination of protein engineering and directed evolution, we will develop SrtA variants capable of efficiently functioning within mammalian cells. We will also develop exceptionally small (
StatusFinished
Effective start/end date9/30/098/31/15

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)