EMPTY CLASS II MHC PROTEINS: TRAFFICKING AND FUNCTION

  • Santambrogio, Laura (PI)

Project: Research project

Project Details

Description

DESCRIPTION (investigator's abstract): The long-term objective of this research
is to characterize the physiological role and relevance in the immunological
response of an alternative pathway of antigen processing and presentation,
known to be active in immature dendritic cells and possibly in central nervous
system microglia. Such alternative pathway relay on abundant cell surface
expression of empty class II MHC protein and H2-DM for peptide loading and
antigen presentation and on secreted dendritic cells proteases for processing
of intact proteins into antigenic peptides. Together, these elements comprised
an unusual extracellular presentation pathway in which antigen processing and
loading occurred entirely outside the cells. The following proposal is aimed to
characterize the trafficking pathway of these empty molecules from their
synthesis and folding in the ER to their appearance at the cell surface; to
understand the developmental regulation of empty class II MHC molecules on
immature dendritic cells and their regulation by the cytokine microenvironment;
and finally, to define the immunological role of empty class II MHC protein
when engaged with T cell receptors expressed on naive, effector and memory
subsets of T cell. The proposed research is part of an interactive research
project grant. The companion project will focus on the biochemical aspects of
antigen processing and peptide loading as part of the characterization of this
novel pathway.
StatusFinished
Effective start/end date1/15/0112/31/04

Funding

  • National Institute of Allergy and Infectious Diseases: $292,250.00

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