EFFECT OF RETROVIRAL INFECTION ON BONE MARROW

Project: Research project

Project Details

Description

The long-term goal of this project is to determine the mechanisms
whereby retroviral infection affects hemopoiesis. A murine
model system has been developed in which the WB91-GV
retrovirus infects adult mice resulting in immunosuppression and
eventually leukemia. WB91-GV is similar to HIV in that it does
not express a v-onc gene and it infects adults animals. In these
studies the effect of retroviral infection on hemopoiesis will be
defined and the temporal relationship of the development of
immunodeficiency to altered hemopoiesis will be established. The
types of bond marrow cells that are infected by WB91-GV will be
determined and the responsiveness of infected cells to GM-CSF,
IL3, and combinations of IL3 plus G-CSF, M-CSF or erythropoietin
will be assessed. The effect of in vivo treatment of infected
animals with GM-CSF in the presence or absence of T
lymphocytes will be examined. Diverse technologies ranging from
in vivo animal studies to molecular analysis of gene expression
will be used. These studies should give us insignt into the effect
of retroviral infection on hemopoiesis and should give us
experience on how to use exogenous growth factors to reserve
some of these effects.
StatusFinished
Effective start/end date1/1/901/1/90

Funding

  • National Heart, Lung, and Blood Institute

ASJC

  • Microbiology (medical)
  • Infectious Diseases
  • Molecular Medicine
  • Virology
  • Immunology

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