Digital Imaging System for Use in Multi-User Facility

Project: Research project

Project Details


DESCRIPTION (provided by applicant): The equipment we are applying for would
provide rapidly switchable attenuated and properly filtered light for wide
field epifluorescence, microscopy in an environmental chamber to preserve
integrity of cultures, high speed low noise detection, off-line storage and
processing of large datasets, deconvolution software to improve spatial
resolution, and image analysis.
The users assembled in this application share a common need and interest in
imaging dynamics of proteins (or nucleic acids) at high temporal and spatial
resolution in live cell cultures. This type of imaging cannot be done at AECOM
currently forcing projects that require this capability to acquire the data by
more indirect and problematic approaches. We demonstrate that new high speed
and lower noise instrumentation currently available obviates technical problems
associated with these projects and will allow the user group to advance their
research more rapidly and with fewer artifacts. The users? projects are varied
and involve the visualization of roles of P1 4-kinases in lysosomal enzyme
sorting (Backer); vesicular trafficking in hepatocytes and oligodenrocytes
(Wolkoff and Larocca); regulation of meiotic recombination (Cohen); roles of
cofilin and L1M-kinase in actin nucleation (Condeelis); and mechanisms of HIV-1
replication (Kalpana). All projects require the capability to image cells or in
vitro assay systems at high spatial resolution with minimum exposure to light
or at high speeds.
Effective start/end date7/1/026/30/03


  • Signal Processing