CORE--FLOW CYTOMETRY AND FACS

Project: Research project

Project Details

Description

The CFAR Flow Cytometry/FACS Core provides state-of-the-art technical and
methodologic support and instrumentation in flow cytometric
analysis/immunophenotyping and fluorescence activated cell sorting for
AIDS investigators at AECOM and collaborating institutions. The FACS
Core lab and personnel have nearly a decade of experience in flow
cytometric immunophenotype analysis of AIDS patients, and has been a
member of the NIAID/DAIDS Flow Cytometry Advisory Committee since 1988.
The Core lab has been fully certified in immunophenotype analysis for
ACTG specimen processing since the inception of the certification
program, and currently serves as the Flow Cytometry lab for seven outside
Pediatric ACTG sites in addition to AECOM Adult and Pediatric ACTG
specimens. The Core lab also serves as a reference lab for the T-cell
determination quality control program of the CDC. The Core lab carries
out fluorochrome staining, analysis and sorting on a wide variety of
research and clinical specimens, including peripheral blood, semen, bone
marrow, mammalian cell lines, and isolated and primary cultures of cells
from fetal tissue. In addition, the Core lab and its personnel are
involved in collaborative efforts to develop flow cytometric procedures
for the evaluation of cellular activation and proliferation, apart from
immunophenotype analysis, as well as methodology for concurrent analysis
of surface phenotype and intracellular HIV-1 p24. The CFAR Flow
Cytometry-FACS Core provides access to quality-controlled analysis of
single-dual, and triple-fluorochrome labelled specimens, and the highest
quality instrumentation for both analysis and sorting. Because the CFAR
Flow/FACS Core is housed within the Institutional P-3 Biohazard Facility,
it also provides a unique resource for analysis/sorting of unfixed cells,
which can only be carried out in a P-3 environment. The Flow
Cytometry/FACS Core has demonstrated ability and capacity for efficiently
handling a large and diverse research and clinical specimen load, as
reflected in its support of numerous AECOM investigators as well as
multiple ACTG sites. In addition, the broad range of experience and
expertise of Core personnel in both research and clinical applications
of Flow Cytometry. provides an effective foundation for promotion of
interactive multidisciplinary collaborations.
StatusFinished
Effective start/end date1/1/012/29/00

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Virology
  • Immunology
  • Pediatrics, Perinatology, and Child Health
  • Cell Biology

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