CARBOHYDRATE AND GLYCOPROTEIN INTERACTIONS WITH LECTINS

Project: Research project

Project Details

Description

DESCRIPTION: (provided by applicant): the oligosaccharide chains of
glycoproteins and glycolipids of normal and transformed cells have been shown
to be receptors in a variety of biological processes, including cellular
recognition, adhesion, apoptosis, differentiation and oncogenic transformation.
Many of these biological effects are due to the interaction of glycoconjugate
receptors with lectins which are carbohydrate binding proteins. The multivalent
binding properties of lectins often results in cross-linking and aggregation of
cell surface glycoconjugate receptors and concomitant signal transduction
effects. Molecular and structural studies have shown that certain lectins form
homogeneous cross-linked complexes with specific multivalent oligosaccharides
and glycoproteins, even in the presence of mixtures of the molecules. Recent
x-ray crystallographic studies have demonstrated the formation of unique
crystalline 2- and 3-dimensional cross-linked lattices between lectins and a
series of multivalent carbohydrates. Moe recently it has been observed that
several specific glycoprotein receptors on the surface of human T cells undergo
separation and selective clustering by the binding and cross-linking of
galectin-1, an endogenous dimeric lectin, resulting in cell death. The
galectin-1 induced separation andselective clustering of different counter
receptors which are associated with phosphatase or kinase activities was
modeled using our molecular studies of lectin-carbohydrate cross-linking
interactions. These and other observations suggest that the selective
cross-linking properties of galectin-1 and other members of the galectin family
are important in their biological activities. The goal of this proposal is to
determine the fine carbohydrate binding specificities, cross-linking and
physical properties of galectins and related lectins in order to understand
their structure-activity properties in normal and transformed cells.
StatusFinished
Effective start/end date1/1/9011/30/08

Funding

  • National Cancer Institute
  • National Cancer Institute: $65,916.00
  • National Cancer Institute
  • National Cancer Institute: $395,466.00
  • National Cancer Institute
  • National Cancer Institute: $374,962.00
  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute: $459,361.00
  • National Cancer Institute: $407,277.00
  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute

ASJC

  • Radiation
  • Spectroscopy
  • Cell Biology

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