ASSEMBLY AND FUNCTION OF THE HERPES SIMPLEX ENVELOPE

Project: Research project

Project Details

Description

Formation of the herpes simplex virus (HSV) envelope, and egress
of enveloped virions from cells, is critical for the production of infectious
particles and the spread of disease. Unfortunately, little is know of how HSV
capsids and tegument proteins refashion cell membranes into envelopes.
Similarly, the identity of the cytoplasmic organelles utilized for viral
exocytosis remains obscure. Our working model is that HSV capsids acquire their
final envelope in the cell cytoplasm by attaching to the surface of the Golgi
apparatus or endosomes, then budding into them. Using novel assay techniques
developed in this laboratory, we will purify then establish the identity and
function of cytoplasmic organelles utilized for HSV envelopment. The mechanism
of association of capsids and tegument proteins with the surface of these
organelles, the first step in viral envelopment, will then be determined.
Finally, the interaction of capsids with cytoplasmic compartments will be
visualized in living cells by imaging the trafficking of Green Fluorescent
Protein tagged capsids during a single, synchronized wave of assembly.
Information obtained from this study will help in the design of agents able to
interfere with HSV envelopment, thus reducing the virulence of this serious
human pathogen.
StatusFinished
Effective start/end date3/1/962/28/05

ASJC

  • Cancer Research
  • Cell Biology
  • Immunology

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