Applications of Click Chemistry in Glycobiology

  • Wu, Peng (PI)

Project: Research project

Project Details

Description

Cell surface glycans are major determinants of cell-cell interactions. Changes In cell surface glycosylation
mark the onset of cancer and inflammation. Inside the cell, they can regulate transcription, translation as well
as protein trafficking. Progress toward delineating the molecular basis of glycan function, however, has been
rather slow. This is partly due to the fact that the biosynthesis of glycans, unlike other biopolymers, is neither
template-driven nor under direct transcriptional control. Therefore, conventional genetic and biochemical
approaches for elucidating glycan function, and its relevance to disease, have yielded limited information.
The long term goal of this project is to implement click chemistry-a set of powerful, reliable and selective
reactions-as a general tool for fundamental studies of glycobiology.
With the experience and knowledge gained from the K99 phase, I will expand my research in two new
directions in the next granting period (ROO). Aim 1 is to discover/develop small molecule inhibitors of glycan
biosynthetic and processing enzymes using enzyme-templated in situ click chemistry. For proof of principle, I
chose 0-beta-N-acetylglucosamlnyl-transferase (OGT) as the first target. I plan to develop fragment libraries
that will be screened for self-assembled inhibitors of OGT. Given the correlation of excessive 0-GlcNAc
modification with prolonged hyperglycemia, which in turn triggers insulin resistance, the compounds
developed may have applications in diabetes therapy. Aim 2 is to intercept glycan biosynthetic pathways with
synthetic unnatural substrates bearing bioorthogonal functional groups, such as azides and alkynes. In
parallel, I will also develop new selective reactions based on click chemistry for their subsequent detection in
live cells.
StatusFinished
Effective start/end date6/19/095/31/12

Funding

  • National Institute of General Medical Sciences: $249,000.00
  • National Institute of General Medical Sciences: $246,510.00
  • National Institute of General Medical Sciences: $244,045.00

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