ANALYSIS OF CW EPR OF HEMOPROTEINS

  • McMillin, David (PI)
  • Coffino, Alan (PI)
  • Fee, James A. (PI)
  • Stafford, Darrel D.W (PI)
  • Kozarich, John (PI)

Project: Research project

Project Details

Description

Continuous wave EPR spectroscopy was used to identify radicals formed during oxygen inactivation of the enzyme, pyruvate formate lyase. Evidence was found for formation of a sulfinyl radical on cysteine in the wild-type enzyme, and in mutant C418A while an alkyl
StatusFinished
Effective start/end date10/1/899/30/99

Funding

  • National Center for Research Resources

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  • BIOTECHNOLOGY RESOURCE IN PULSED EPR SPECTROSCOPY

    Mildvan, A. A., Nersissian, A. A., Lee, C. C. C., Stafford, D. D. W., Singel, D. D., Ash, D. D. E., Janson, E. E., Guajardo, G. G. B., Dyson, H. H. J., Peisach, J. J., Wittaker, J. J., Fee, J. J. A., Kozarich, J. J., Shanklin, J. J., Chan, J. J., Sam, J. J., Colaneri, M. M., Walter, P. P., Danzinger, R. R., Banerjee, R. R. V., Schramm, V. V. L., Wang, W. W., Mims, W. W. B., Peisach, J., Aisen, P., Armstrong, W. H., Astroff, N., Avdievich, N., Baker, T. A., Bender, C., Benkovic, S., Brewer, C. F., Brewer, F., Britt, D., Cammack, R., Caradonna, J., Chan, J., Colaneri, M., Das, T., Dooley, D., Dyson, H. J., Ettinger, M., Fabry, M. E., Fee, J. A., Frank, N. H. A., Friedman, J. M., Gerfen, G. J., Goutermann, M., Kamp, N., Kozarich, J., Lee, C. C., Lippai, I., Magliozzo, R. S., Mason, R., McMillin, D., Michael, D., Mims, W. B., Narasimhulu, S., Ohnishi, T., Palmer, G., Que, L., Rousseau, D. L., Sam, J., Scholes, C., Scholes, C. P., Shaka, O., Sinclair, P., Singel, D., Stegeman, J., Stubbe, N. J. A., Stubbe, J., Tipton, P. A., Villafranca, N. J. J., Wang, W., Zweier, J. L., Belford, L., Beltramini, M., Chance, M., Clarkson, R., Coffino, A., Crowder, M., Ranney, H. H., Klinman, J. J., Klein, M., Klinman, J., Krzystek, Y., Licht, S., Marcinkeviciene, J., Gluck, M. M. R., Mcdermott, A., Bowman, M. M., Ming, L., Sumuni, R. R., Sands, R., Sheik, A., Stubbe, J., Tsuneshige, A., Valentine, J., Vieso, V., Wirt, M., Wong, K. & Zhang, C.

    9/30/854/30/00

    Project: Research project