• 13071 Citations
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1960 …2023

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Article
2020

CLL intraclonal fractions exhibit established and recently acquired patterns of DNA methylation

Bartholdy, B. A., Wang, X., Yan, X. J., Pascual, M., Fan, M., Barrientos, J., Allen, S. L., Martinez-Climent, J. A., Rai, K. R., Chiorazzi, N., Scharff, M. D. & Roa, S., Mar 10 2020, In : Blood Advances. 4, 5, p. 893-906 14 p.

Research output: Contribution to journalArticle

Open Access
2018

Human immunodeficiency virus tat protein aids V region somatic hypermutation in human B cells

Wang, X., Duan, Z., Yu, G., Fan, M. & Scharff, M. D., Mar 1 2018, In : mBio. 9, 2, e02315-17.

Research output: Contribution to journalArticle

2015

Overlapping hotspots in CDRs are critical sites for V region diversification

Wei, L., Chahwan, R., Wang, S., Wang, X., Pham, P. T., Goodman, M. F., Bergman, A., Scharff, M. D. & MacCarthy, T., Feb 17 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 7, p. E728-E737

Research output: Contribution to journalArticle

24 Scopus citations
2014

A source of the single-stranded DNA substrate for activation-induced deaminase during somatic hypermutation

Wang, X., Fan, M., Kalis, S., Wei, L. & Scharff, M. D., Jun 13 2014, In : Nature communications. 5, 4137.

Research output: Contribution to journalArticle

18 Scopus citations

Detection and measurement of staphylococcal enterotoxin-like K (SEl-K) secretion by Staphylococcus aureus clinical isolates

Aguilar, J. L., Varshney, A. K., Wang, X., Stanford, L., Scharff, M. & Fries, B. C., Jul 2014, In : Journal of Clinical Microbiology. 52, 7, p. 2536-2543 8 p.

Research output: Contribution to journalArticle

14 Scopus citations

Isotype switching increases efficacy of antibody protection against staphylococcal enterotoxin B-induced lethal shock and Staphylococcus aureus sepsis in mice

Varshney, A. K., Wang, X., Aguilar, J. L., Scharff, M. D. & Fries, B. C., Jun 10 2014, In : mBio. 5, 3

Research output: Contribution to journalArticle

25 Scopus citations

The MutSβ complex is a modulator of p53-driven tumorigenesis through its functions in both DNA double-strand break repair and mismatch repair

Van Oers, J. M. M., Edwards, Y., Chahwan, R., Zhang, W., Smith, C., Pechuan, X., Schaetzlein, S., Jin, B., Wang, Y., Bergman, A., Scharff, M. D. & Edelmann, W., Jul 24 2014, In : Oncogene. 33, 30, p. 3939-3946 8 p.

Research output: Contribution to journalArticle

16 Scopus citations
2013

HHLA2 is a member of the B7 family and inhibits human CD4 and CD8 T-cell function

Zhao, R., Chinai, J. M., Buhl, S., Scandiuzzi, L., Ray, A., Jeon, H., Ohaegbulam, K. C., Ghosh, K., Zhao, A., Scharff, M. D. & Zang, X., Jun 11 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 24, p. 9879-9884 6 p.

Research output: Contribution to journalArticle

61 Scopus citations

Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes

Schaetzlein, S., Chahwan, R., Avdievich, E., Roa, S., Wei, K., Eoff, R. L., Sellers, R. S., Clark, A. B., Kunkelf, T. A., Scharff, M. D. & Edelmann, W., Jul 2 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 27, p. E2470-E2479

Research output: Contribution to journalArticle

37 Scopus citations

Staphylococcal enterotoxin B-specific monoclonal antibody 20B1 successfully treats diverse staphylococcus aureus infections

Varshney, A. K., Wang, X., Scharff, M. D., Maclntyre, J., Zollner, R. S., Kovalenko, O. V., Martinez, L. R., Byrne, F. R. & Fries, B. C., Dec 15 2013, In : Journal of Infectious Diseases. 208, 12, p. 2058-2066 9 p.

Research output: Contribution to journalArticle

30 Scopus citations
2012

Germline deletion of Igh 3′ regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus

Volpi, S. A., Verma-Gaur, J., Hassan, R., Ju, Z., Roa, S., Chatterjee, S., Werling, U., Hou, H., Will, B., Steidl, U., Scharff, M., Edelman, W., Feeney, A. J. & Birshtein, B. K., Mar 15 2012, In : Journal of Immunology. 188, 6, p. 2556-2566 11 p.

Research output: Contribution to journalArticle

28 Scopus citations

IGHV-unmutated and IGHV-mutated chronic lymphocytic leukemia cells produce activation-induced deaminase protein with a full range of biologic functions

Patten, P. E. M., Chu, C. C., Albesiano, E., Damle, R. N., Yan, X. J., Kim, D., Zhang, L., Magli, A. R., Barrientos, J., Kolitz, J. E., Allen, S. L., Rai, K. R., Roa, S., Mongini, P. K., MacCarthy, T., Scharff, M. D. & Chiorazzi, N., Dec 6 2012, In : Blood. 120, 24, p. 4802-4811 10 p.

Research output: Contribution to journalArticle

35 Scopus citations

The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination

Chahwan, R., van Oers, J. M. M., Avdievich, E., Zhao, C., Edelmann, W., Scharff, M. D. & Roa, S. R., Apr 9 2012, In : Journal of Experimental Medicine. 209, 4, p. 671-678 8 p.

Research output: Contribution to journalArticle

17 Scopus citations

The use of Bcl-2 over-expression to stabilize hybridomas specific to the HERG potassium channel

Sroubek, J., Krishnan, Y., Chinai, J., Buhl, S., Scharff, M. D. & McDonald, T. V., Jan 31 2012, In : Journal of Immunological Methods. 375, 1-2, p. 215-222 8 p.

Research output: Contribution to journalArticle

2 Scopus citations
2011

Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin B-induced lethal shock

Varshney, A. K., Wang, X., Cook, E., Dutta, K., Scharff, M. D., Goger, M. J. & Fries, B. C., Mar 18 2011, In : Journal of Biological Chemistry. 286, 11, p. 9737-9747 11 p.

Research output: Contribution to journalArticle

43 Scopus citations

Intraclonal complexity in chronic lymphocytic leukemia: Fractions enriched in recently born/divided and older/quiescent cells

Calissano, C., Damle, R. N., Marsilio, S., Yan, X. J., Yancopoulos, S., Hayes, G., Emson, C., Murphy, E. J., Hellerstein, M. K., Sison, C., Kaufman, M. S., Kolitz, J. E., Allen, S. L., Rai, K. R., Ivanovic, I., Dozmorov, I. M., Roa, S., Scharff, M. D., Li, W. & Chiorazzi, N., Nov 1 2011, In : Molecular Medicine. 17, 11, p. 1374-1382 9 p.

Research output: Contribution to journalArticle

69 Scopus citations

Mismatch-mediated error prone repair at the immunoglobulin genes

Chahwan, R., Edelmann, W., Scharff, M. D. & Roa, S., Dec 1 2011, In : Biomedicine and Pharmacotherapy. 65, 8, p. 529-536 8 p.

Research output: Contribution to journalArticle

14 Scopus citations

Recombinase-mediated cassette exchange as a novel method to study somatic hypermutation in ramos cells

Baughn, L. B., Kalis, S. L., MacCarthy, T., Wei, L., Fan, M., Bergman, A. & Scharff, M. D., Sep 1 2011, In : mBio. 2, 5

Research output: Contribution to journalArticle

6 Scopus citations
2010

A requirement for FcγR in antibody-mediated bacterial toxin neutralization

Abboud, N., Chow, S. K., Saylor, C., Janda, A., Ravetch, J. V., Scharff, M. D. & Casadevall, A., Oct 25 2010, In : Journal of Experimental Medicine. 207, 11, p. 2395-2405 11 p.

Research output: Contribution to journalArticle

78 Scopus citations

MSH2/MSH6 complex promotes error-free repair of AID-Induced dU:G mispairs as well as error-prone hypermutation of A:T Sites

Roa, S., Li, Z., Peled, J. U., Zhao, C., Edelmann, W. & Scharff, M. D., Aug 11 2010, In : PloS one. 5, 6, e11182.

Research output: Contribution to journalArticle

28 Scopus citations

Msh6 protects mature B cells from lymphoma by preserving genomic stability

Peled, J. U., Sellers, R. S., Iglesias-Ussel, M. D., Shin, D. M., Montagna, C., Zhao, C., Li, Z., Edelmann, W., Morse, H. C. & Scharff, M. D., Nov 2010, In : American Journal of Pathology. 177, 5, p. 2597-2608 12 p.

Research output: Contribution to journalArticle

9 Scopus citations

PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance

Van Oers, J. M. M., Roa, S., Werling, U., Liu, Y., Genschel, J., Hou, H., Sellers, R. S., Modrich, P., Scharff, M. D. & Edelmann, W., Jul 27 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 30, p. 13384-13389 6 p.

Research output: Contribution to journalArticle

40 Scopus citations

Requirement for cyclin D3 in germinal center formation and function

Peled, J. U., Yu, J. J., Venkatesh, J., Bi, E., Ding, B. B., Krupski-Downs, M., Shaknovich, R., Sicinski, P., Diamond, B., Scharff, M. D. & Ye, B. H., Jun 1 2010, In : Cell Research. 20, 6, p. 631-646 16 p.

Research output: Contribution to journalArticle

39 Scopus citations

The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination

Ramachandran, S., Chahwan, R., Nepal, R. M., Frieder, D., Panier, S., Roa, S., Zaheen, A., Durocher, D., Scharff, M. D. & Martin, A., Feb 15 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 2, p. 809-814 6 p.

Research output: Contribution to journalArticle

51 Scopus citations
2009
44 Scopus citations

Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro

Iglesias-Ussel, M. D., Zavadil, J. & Scharff, M. D., Oct 31 2009, In : Journal of Immunological Methods. 350, 1-2, p. 71-78 8 p.

Research output: Contribution to journalArticle

2 Scopus citations

SHMTool: A webserver for comparative analysis of somatic hypermutation datasets

MacCarthy, T., Roa, S., Scharff, M. D. & Bergman, A., Jan 1 2009, In : DNA Repair. 8, 1, p. 137-141 5 p.

Research output: Contribution to journalArticle

26 Scopus citations

V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment

MacCarthy, T., Kalis, S. L., Roa, S., Pham, P., Goodman, M. F., Scharff, M. D. & Bergman, A., May 26 2009, In : Proceedings of the National Academy of Sciences of the United States of America. 106, 21, p. 8629-8634 6 p.

Research output: Contribution to journalArticle

27 Scopus citations
2008

Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression

Roa, S., Avdievich, E., Peled, J. U., MacCarthy, T., Werling, U., Fei, L. K., Kan, R., Zhao, C., Bergman, A., Cohen, P. E., Edelmann, W. & Scharff, M. D., Oct 21 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 42, p. 16248-16253 6 p.

Research output: Contribution to journalArticle

70 Scopus citations
2007

Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation

Ronai, D., Iglesias-Ussel, M. D., Fan, M., Li, Z., Martin, A. & Scharff, M. D., Jan 22 2007, In : Journal of Experimental Medicine. 204, 1, p. 181-190 10 p.

Research output: Contribution to journalArticle

61 Scopus citations

Targeting AID to the Ig genes.

Li, Z., Luo, Z., Ronai, D., Kuang, F. L., Peled, J. U., Iglesias-Ussel, M. D. & Scharff, M. D., 2007, In : Advances in Experimental Medicine and Biology. 596, p. 93-109 17 p.

Research output: Contribution to journalArticle

2006

A role for Mlh3 in somatic hypermutation

Li, Z., Peled, J. U., Zhao, C., Svetlanov, A., Ronai, D., Cohen, P. E. & Scharff, M. D., Jun 10 2006, In : DNA Repair. 5, 6, p. 675-682 8 p.

Research output: Contribution to journalArticle

20 Scopus citations

Forced expression of AID facilitates the isolation of class switch variants from hybridoma cells

Iglesias-Ussel, M. D., Fan, M., Li, Z., Martin, A. & Scharff, M. D., Oct 30 2006, In : Journal of Immunological Methods. 316, 1-2, p. 59-66 8 p.

Research output: Contribution to journalArticle

4 Scopus citations

The Mismatch Repair Protein Msh6 Influences the In Vivo AID Targeting to the Ig Locus

Li, Z., Zhao, C., Iglesias-Ussel, M. D., Polonskaya, Z., Zhuang, M., Yang, G., Luo, Z., Edelmann, W. & Scharff, M. D., Apr 1 2006, In : Immunity. 24, 4, p. 393-403 11 p.

Research output: Contribution to journalArticle

35 Scopus citations

Tumor necrosis factor-α and CD40L modulate cell surface morphology and induce aggregation in Ramos Burkitt's lymphoma cells

Laskov, R., Berger, N., Scharff, M. D. & Horwitz, M. S., Mar 1 2006, In : Leukemia and Lymphoma. 47, 3, p. 507-519 13 p.

Research output: Contribution to journalArticle

3 Scopus citations
2005

Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells

Ronai, D., Iglesias-Ussel, M. D., Fan, M., Shulman, M. J. & Scharff, M. D., Aug 16 2005, In : Proceedings of the National Academy of Sciences of the United States of America. 102, 33, p. 11829-11834 6 p.

Research output: Contribution to journalArticle

20 Scopus citations

Methylation protects cytidines from AID-mediated deamination

Larijani, M., Frieder, D., Sonbuchner, T. M., Bransteitter, R., Goodman, M. F., Bouhassira, E. E., Scharff, M. D. & Martin, A., Mar 1 2005, In : Molecular Immunology. 42, 5, p. 599-604 6 p.

Research output: Contribution to journalArticle

57 Scopus citations

Variable-region-identical antibodies differing in isotype demonstrate differences in fine specificity and idiotype

Torres, M., May, R., Scharff, M. D. & Casadevall, A., Feb 15 2005, In : Journal of Immunology. 174, 4, p. 2132-2142 11 p.

Research output: Contribution to journalArticle

Open Access
63 Scopus citations
2004

Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice

Bardwell, P. D., Woo, C. J., Wei, K., Li, Z., Martin, A., Sack, S. Z., Parris, T., Edelmann, W. & Scharff, M. D., Feb 1 2004, In : Nature Immunology. 5, 2, p. 224-229 6 p.

Research output: Contribution to journalArticle

196 Scopus citations
46 Scopus citations

Examination of Msh6- and Msh3-deficient mice in class switching reveals overlapping and distinct roles of MutS homologues in antibody diversification

Li, Z., Scherer, S. J., Ronai, D., Iglesias-Ussel, M. D., Peled, J. U., Bardwell, P. D., Zhuang, M., Lee, K. R., Martin, A., Edelmann, W. & Scharff, M. D., Jul 5 2004, In : Journal of Experimental Medicine. 200, 1, p. 47-59 13 p.

Research output: Contribution to journalArticle

83 Scopus citations
2003

Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase

Bransteitter, R., Pham, P., Scharfft, M. D. & Goodman, M. F., Apr 1 2003, In : Proceedings of the National Academy of Sciences of the United States of America. 100, 7, p. 4102-4107 6 p.

Research output: Contribution to journalArticle

525 Scopus citations
Open Access
34 Scopus citations

Cutting edge: The G-U mismatch glycosylase methyl-CpG binding domain 4 is dispensable for somatic hypermutation and class switch recombination

Bardwell, P. D., Martin, A., Wong, E., Li, Z., Edelmann, W. & Scharff, M. D., Feb 15 2003, In : Journal of Immunology. 170, 4, p. 1620-1624 5 p.

Research output: Contribution to journalArticle

Open Access
39 Scopus citations

Induction of somatic hypermutation is associated with modifications in immunoglobulin variable region chromatin

Woo, C. J., Martin, A. & Scharff, M. D., Oct 2003, In : Immunity. 19, 4, p. 479-489 11 p.

Research output: Contribution to journalArticle

85 Scopus citations

Msh2 ATPase Activity Is Essential for Somatic Hypermutation at A-T Basepairs and for Efficient Class Switch Recombination

Martin, A., Li, Z., Lin, D. P., Bardwell, P. D., Iglesias-Ussel, M. D., Edelmann, W. & Scharff, M. D., Oct 20 2003, In : Journal of Experimental Medicine. 198, 8, p. 1171-1178 8 p.

Research output: Contribution to journalArticle

77 Scopus citations
2002

Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas

Martin, A., Bardwell, P. D., Woo, C. J., Fan, M., Shulman, M. J. & Scharff, M. D., Feb 14 2002, In : Nature. 415, 6873, p. 802-806 5 p.

Research output: Contribution to journalArticle

216 Scopus citations

Fcγ receptor I- and III-mediated macrophage inflammatory protein 1α induction in primary human and murine microglia

Song, X., Shapiro, S., Goldman, D. L., Casadevall, A., Scharff, M. & Lee, S. C., Sep 1 2002, In : Infection and immunity. 70, 9, p. 5177-5184 8 p.

Research output: Contribution to journalArticle

45 Scopus citations

High affinity mimotope of the polysaccharide capsule of Cryptococcus neoformans identified from an evolutionary phage peptide library

Beenhouwer, D. O., May, R. J., Valadon, P. & Scharff, M. D., Dec 15 2002, In : Journal of Immunology. 169, 12, p. 6992-6999 8 p.

Research output: Contribution to journalArticle

Open Access
53 Scopus citations

Immunoglobulin G monoclonal antibodies to Cryptococcus neoformans protect mice deficient in complement component C3

Shapiro, S., Beenhouwer, D. O., Feldmesser, M., Taborda, C., Carroll, M. C., Casadevall, A. & Scharff, M. D., Apr 29 2002, In : Infection and immunity. 70, 5, p. 2598-2604 7 p.

Research output: Contribution to journalArticle

62 Scopus citations