• 13181 Citations
  • 62 h-Index
1960 …2020

Research output per year

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Publications

2020

AID Overlapping and Polη Hotspots Are Key Features of Evolutionary Variation Within the Human Antibody Heavy Chain (IGHV) Genes

Tang, C., Bagnara, D., Chiorazzi, N., Scharff, M. D. & MacCarthy, T., Apr 30 2020, In : Frontiers in immunology. 11, 788.

Research output: Contribution to journalArticle

Open Access

CLL intraclonal fractions exhibit established and recently acquired patterns of DNA methylation

Bartholdy, B. A., Wang, X., Yan, X. J., Pascual, M., Fan, M., Barrientos, J., Allen, S. L., Martinez-Climent, J. A., Rai, K. R., Chiorazzi, N., Scharff, M. D. & Roa, S., Mar 10 2020, In : Blood Advances. 4, 5, p. 893-906 14 p.

Research output: Contribution to journalArticle

Open Access
2018

Human immunodeficiency virus tat protein aids V region somatic hypermutation in human B cells

Wang, X., Duan, Z., Yu, G., Fan, M. & Scharff, M. D., Mar 1 2018, In : mBio. 9, 2, e02315-17.

Research output: Contribution to journalArticle

2016

Error-Prone Mismatch and Base Excision DNA Repair in Somatic Hypermutation

Wang, S., Chahwan, R., Wei, L. & Scharff, M. D., Apr 27 2016, Molecular Immunology. Elsevier Inc., Vol. 2. p. 126-133 8 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2015

Overlapping hotspots in CDRs are critical sites for V region diversification

Wei, L., Chahwan, R., Wang, S., Wang, X., Pham, P. T., Goodman, M. F., Bergman, A., Scharff, M. D. & MacCarthy, T., Feb 17 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 7, p. E728-E737

Research output: Contribution to journalArticle

25 Scopus citations
2014

A source of the single-stranded DNA substrate for activation-induced deaminase during somatic hypermutation

Wang, X., Fan, M., Kalis, S., Wei, L. & Scharff, M. D., Jun 13 2014, In : Nature communications. 5, 4137.

Research output: Contribution to journalArticle

19 Scopus citations

Detection and measurement of staphylococcal enterotoxin-like K (SEl-K) secretion by Staphylococcus aureus clinical isolates

Aguilar, J. L., Varshney, A. K., Wang, X., Stanford, L., Scharff, M. & Fries, B. C., Jul 2014, In : Journal of Clinical Microbiology. 52, 7, p. 2536-2543 8 p.

Research output: Contribution to journalArticle

15 Scopus citations

Isotype switching increases efficacy of antibody protection against staphylococcal enterotoxin B-induced lethal shock and Staphylococcus aureus sepsis in mice

Varshney, A. K., Wang, X., Aguilar, J. L., Scharff, M. D. & Fries, B. C., Jun 10 2014, In : mBio. 5, 3, e01007-14.

Research output: Contribution to journalArticle

25 Scopus citations

Somatic Hypermutation: The Molecular Mechanisms Underlying the Production of Effective High-Affinity Antibodies. The Molecular Mechanisms Underlying the Production of Effective High-Affinity Antibodies.

Martin, A., Chahwan, R., Parsa, J. Y. & Scharff, M. D., Dec 15 2014, Molecular Biology of B Cells: Second Edition. Elsevier Inc., p. 363-388 26 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

The MutSβ complex is a modulator of p53-driven tumorigenesis through its functions in both DNA double-strand break repair and mismatch repair

Van Oers, J. M. M., Edwards, Y., Chahwan, R., Zhang, W., Smith, C., Pechuan, X., Schaetzlein, S., Jin, B., Wang, Y., Bergman, A., Scharff, M. D. & Edelmann, W., Jul 24 2014, In : Oncogene. 33, 30, p. 3939-3946 8 p.

Research output: Contribution to journalArticle

16 Scopus citations
2013

AID and Apobec3G haphazard deamination and mutational diversity

Jaszczur, M., Bertram, J. G., Pham, P., Scharff, M. D. & Goodman, M. F., Sep 2013, In : Cellular and Molecular Life Sciences. 70, 17, p. 3089-3108 20 p.

Research output: Contribution to journalReview article

20 Scopus citations

HHLA2 is a member of the B7 family and inhibits human CD4 and CD8 T-cell function

Zhao, R., Chinai, J. M., Buhl, S., Scandiuzzi, L., Ray, A., Jeon, H., Ohaegbulam, K. C., Ghosh, K., Zhao, A., Scharff, M. D. & Zang, X., Jun 11 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 24, p. 9879-9884 6 p.

Research output: Contribution to journalArticle

67 Scopus citations

Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes

Schaetzlein, S., Chahwan, R., Avdievich, E., Roa, S., Wei, K., Eoff, R. L., Sellers, R. S., Clark, A. B., Kunkelf, T. A., Scharff, M. D. & Edelmann, W., Jul 2 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 27, p. E2470-E2479

Research output: Contribution to journalArticle

38 Scopus citations

Staphylococcal enterotoxin B-specific monoclonal antibody 20B1 successfully treats diverse staphylococcus aureus infections

Varshney, A. K., Wang, X., Scharff, M. D., Maclntyre, J., Zollner, R. S., Kovalenko, O. V., Martinez, L. R., Byrne, F. R. & Fries, B. C., Dec 15 2013, In : Journal of Infectious Diseases. 208, 12, p. 2058-2066 9 p.

Research output: Contribution to journalArticle

31 Scopus citations
2012

AIDing antibody diversity by error-prone mismatch repair

Chahwan, R., Edelmann, W., Scharff, M. D. & Roa, S., Aug 1 2012, In : Seminars in Immunology. 24, 4, p. 293-300 8 p.

Research output: Contribution to journalReview article

44 Scopus citations

Germline deletion of Igh 3′ regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus

Volpi, S. A., Verma-Gaur, J., Hassan, R., Ju, Z., Roa, S., Chatterjee, S., Werling, U., Hou, H., Will, B., Steidl, U., Scharff, M., Edelman, W., Feeney, A. J. & Birshtein, B. K., Mar 15 2012, In : Journal of Immunology. 188, 6, p. 2556-2566 11 p.

Research output: Contribution to journalArticle

30 Scopus citations

IGHV-unmutated and IGHV-mutated chronic lymphocytic leukemia cells produce activation-induced deaminase protein with a full range of biologic functions

Patten, P. E. M., Chu, C. C., Albesiano, E., Damle, R. N., Yan, X. J., Kim, D., Zhang, L., Magli, A. R., Barrientos, J., Kolitz, J. E., Allen, S. L., Rai, K. R., Roa, S., Mongini, P. K., MacCarthy, T., Scharff, M. D. & Chiorazzi, N., Dec 6 2012, In : Blood. 120, 24, p. 4802-4811 10 p.

Research output: Contribution to journalArticle

35 Scopus citations

The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination

Chahwan, R., van Oers, J. M. M., Avdievich, E., Zhao, C., Edelmann, W., Scharff, M. D. & Roa, S. R., Apr 9 2012, In : Journal of Experimental Medicine. 209, 4, p. 671-678 8 p.

Research output: Contribution to journalArticle

17 Scopus citations

The use of Bcl-2 over-expression to stabilize hybridomas specific to the HERG potassium channel

Sroubek, J., Krishnan, Y., Chinai, J., Buhl, S., Scharff, M. D. & McDonald, T. V., Jan 31 2012, In : Journal of Immunological Methods. 375, 1-2, p. 215-222 8 p.

Research output: Contribution to journalArticle

2 Scopus citations
2011

Generation, characterization, and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin B-induced lethal shock

Varshney, A. K., Wang, X., Cook, E., Dutta, K., Scharff, M. D., Goger, M. J. & Fries, B. C., Mar 18 2011, In : Journal of Biological Chemistry. 286, 11, p. 9737-9747 11 p.

Research output: Contribution to journalArticle

43 Scopus citations

Intraclonal complexity in chronic lymphocytic leukemia: Fractions enriched in recently born/divided and older/quiescent cells

Calissano, C., Damle, R. N., Marsilio, S., Yan, X. J., Yancopoulos, S., Hayes, G., Emson, C., Murphy, E. J., Hellerstein, M. K., Sison, C., Kaufman, M. S., Kolitz, J. E., Allen, S. L., Rai, K. R., Ivanovic, I., Dozmorov, I. M., Roa, S., Scharff, M. D., Li, W. & Chiorazzi, N., Nov 1 2011, In : Molecular Medicine. 17, 11, p. 1374-1382 9 p.

Research output: Contribution to journalArticle

72 Scopus citations

Mismatch-mediated error prone repair at the immunoglobulin genes

Chahwan, R., Edelmann, W., Scharff, M. D. & Roa, S., Dec 1 2011, In : Biomedicine and Pharmacotherapy. 65, 8, p. 529-536 8 p.

Research output: Contribution to journalArticle

14 Scopus citations

Recombinase-mediated cassette exchange as a novel method to study somatic hypermutation in ramos cells

Baughn, L. B., Kalis, S. L., MacCarthy, T., Wei, L., Fan, M., Bergman, A. & Scharff, M. D., 2011, In : mBio. 2, 5, e00186-11.

Research output: Contribution to journalArticle

6 Scopus citations
2010

A requirement for FcγR in antibody-mediated bacterial toxin neutralization

Abboud, N., Chow, S. K., Saylor, C., Janda, A., Ravetch, J. V., Scharff, M. D. & Casadevall, A., Oct 25 2010, In : Journal of Experimental Medicine. 207, 11, p. 2395-2405 11 p.

Research output: Contribution to journalArticle

79 Scopus citations

MSH2/MSH6 complex promotes error-free repair of AID-Induced dU:G mispairs as well as error-prone hypermutation of A:T Sites

Roa, S., Li, Z., Peled, J. U., Zhao, C., Edelmann, W. & Scharff, M. D., 2010, In : PloS one. 5, 6, e11182.

Research output: Contribution to journalArticle

28 Scopus citations

Msh6 protects mature B cells from lymphoma by preserving genomic stability

Peled, J. U., Sellers, R. S., Iglesias-Ussel, M. D., Shin, D. M., Montagna, C., Zhao, C., Li, Z., Edelmann, W., Morse, H. C. & Scharff, M. D., Nov 2010, In : American Journal of Pathology. 177, 5, p. 2597-2608 12 p.

Research output: Contribution to journalArticle

9 Scopus citations

PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance

Van Oers, J. M. M., Roa, S., Werling, U., Liu, Y., Genschel, J., Hou, H., Sellers, R. S., Modrich, P., Scharff, M. D. & Edelmann, W., Jul 27 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 30, p. 13384-13389 6 p.

Research output: Contribution to journalArticle

42 Scopus citations

Requirement for cyclin D3 in germinal center formation and function

Peled, J. U., Yu, J. J., Venkatesh, J., Bi, E., Ding, B. B., Krupski-Downs, M., Shaknovich, R., Sicinski, P., Diamond, B., Scharff, M. D. & Ye, B. H., Jun 2010, In : Cell Research. 20, 6, p. 631-646 16 p.

Research output: Contribution to journalArticle

41 Scopus citations

The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination

Ramachandran, S., Chahwan, R., Nepal, R. M., Frieder, D., Panier, S., Roa, S., Zaheen, A., Durocher, D., Scharff, M. D. & Martin, A., 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 2, p. 809-814 6 p.

Research output: Contribution to journalArticle

51 Scopus citations
2009
45 Scopus citations

Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro

Iglesias-Ussel, M. D., Zavadil, J. & Scharff, M. D., Oct 31 2009, In : Journal of Immunological Methods. 350, 1-2, p. 71-78 8 p.

Research output: Contribution to journalArticle

2 Scopus citations

SHMTool: A webserver for comparative analysis of somatic hypermutation datasets

MacCarthy, T., Roa, S., Scharff, M. D. & Bergman, A., Jan 1 2009, In : DNA Repair. 8, 1, p. 137-141 5 p.

Research output: Contribution to journalArticle

26 Scopus citations

V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment

MacCarthy, T., Kalis, S. L., Roa, S., Pham, P., Goodman, M. F., Scharff, M. D. & Bergman, A., May 26 2009, In : Proceedings of the National Academy of Sciences of the United States of America. 106, 21, p. 8629-8634 6 p.

Research output: Contribution to journalArticle

29 Scopus citations
2008

Does antisense make sense of AID targeting?

Roa, S., Fei, L. K. & Scharff, M. D., Mar 25 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 10, p. 3661-3662 2 p.

Research output: Contribution to journalComment/debate

8 Scopus citations

Human recombinant Fab fragments with sub-nanomolar affinities for acetylated histones

Batova, I., Kowal, C., May, R., Scharff, M. D. & Diamond, B., Jan 1 2008, In : Journal of Immunological Methods. 329, 1-2, p. 1-10 10 p.

Research output: Contribution to journalReview article

3 Scopus citations

The biochemistry of somatic hypermutation

Peled, J. U., Fei, L. K., Iglesias-Ussel, M. D., Roa, S., Kalis, S. L., Goodman, M. F. & Scharff, M. D., May 5 2008, In : Annual Review of Immunology. 26, p. 481-511 31 p.

Research output: Contribution to journalReview article

329 Scopus citations

Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression

Roa, S., Avdievich, E., Peled, J. U., MacCarthy, T., Werling, U., Fei, L. K., Kan, R., Zhao, C., Bergman, A., Cohen, P. E., Edelmann, W. & Scharff, M. D., Oct 21 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 42, p. 16248-16253 6 p.

Research output: Contribution to journalArticle

73 Scopus citations
2007

AID-Initiated Purposeful Mutations in Immunoglobulin Genes

Goodman, M. F., Scharff, M. D. & Romesberg, F. E., Jun 7 2007, AID for Immunoglobulin Diversity. Alt, F. & Honjo, T. (eds.). p. 127-155 29 p. (Advances in Immunology; vol. 94).

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Scopus citations

Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation

Ronai, D., Iglesias-Ussel, M. D., Fan, M., Li, Z., Martin, A. & Scharff, M. D., Jan 22 2007, In : Journal of Experimental Medicine. 204, 1, p. 181-190 10 p.

Research output: Contribution to journalArticle

61 Scopus citations

Mouse Models revealed the Mechanisms for Somatic Hypermutation and Class Switch Recombination of Immunoglobulin Genes

Iglesias-Ussel, M. D., Ziqiang, L. & Scharff, M. D., Dec 1 2007, The Mouse in Biomedical Research. Elsevier Inc., Vol. 4. p. 155-168 14 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Targeting AID to the Ig genes

Li, Z., Luo, Z., Ronai, D., Li Kuang, F., Peled, J. U., Iglesias-Ussel, M. D. & Scharff, M. D., Jan 1 2007, Mechanisms of Lymphocyte Activation and Immune Regulation XI: B Cell Biology. Springer New York, p. 93-109 17 p. (Advances in Experimental Medicine and Biology; vol. 596).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Targeting AID to the Ig genes.

Li, Z., Luo, Z., Ronai, D., Kuang, F. L., Peled, J. U., Iglesias-Ussel, M. D. & Scharff, M. D., 2007, In : Advances in Experimental Medicine and Biology. 596, p. 93-109 17 p.

Research output: Contribution to journalArticle

2006

A role for Mlh3 in somatic hypermutation

Li, Z., Peled, J. U., Zhao, C., Svetlanov, A., Ronai, D., Cohen, P. E. & Scharff, M. D., Jun 10 2006, In : DNA Repair. 5, 6, p. 675-682 8 p.

Research output: Contribution to journalArticle

20 Scopus citations

Forced expression of AID facilitates the isolation of class switch variants from hybridoma cells

Iglesias-Ussel, M. D., Fan, M., Li, Z., Martin, A. & Scharff, M. D., Oct 30 2006, In : Journal of Immunological Methods. 316, 1-2, p. 59-66 8 p.

Research output: Contribution to journalArticle

4 Scopus citations

The Mismatch Repair Protein Msh6 Influences the In Vivo AID Targeting to the Ig Locus

Li, Z., Zhao, C., Iglesias-Ussel, M. D., Polonskaya, Z., Zhuang, M., Yang, G., Luo, Z., Edelmann, W. & Scharff, M. D., Apr 1 2006, In : Immunity. 24, 4, p. 393-403 11 p.

Research output: Contribution to journalArticle

35 Scopus citations

Tumor necrosis factor-α and CD40L modulate cell surface morphology and induce aggregation in Ramos Burkitt's lymphoma cells

Laskov, R., Berger, N., Scharff, M. D. & Horwitz, M. S., Mar 1 2006, In : Leukemia and Lymphoma. 47, 3, p. 507-519 13 p.

Research output: Contribution to journalArticle

3 Scopus citations
2005

Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells

Ronai, D., Iglesias-Ussel, M. D., Fan, M., Shulman, M. J. & Scharff, M. D., Aug 16 2005, In : Proceedings of the National Academy of Sciences of the United States of America. 102, 33, p. 11829-11834 6 p.

Research output: Contribution to journalArticle

20 Scopus citations

Identifying protein-protein interactions in somatic hypermutation

Goodman, M. F. & Scharff, M. D., Feb 21 2005, In : Journal of Experimental Medicine. 201, 4, p. 493-496 4 p.

Research output: Contribution to journalReview article

5 Scopus citations

Methylation protects cytidines from AID-mediated deamination

Larijani, M., Frieder, D., Sonbuchner, T. M., Bransteitter, R., Goodman, M. F., Bouhassira, E. E., Scharff, M. D. & Martin, A., Mar 1 2005, In : Molecular Immunology. 42, 5, p. 599-604 6 p.

Research output: Contribution to journalArticle

58 Scopus citations